H4409
EX-CELL® CD Hybridoma Medium, Chemically Defined
Animal-component free, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for hybridoma, suitable for cell culture
Synonym(s):
Hybridoma Medium
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About This Item
₹75,396.40
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Quality Level
description
for research or for further manufacturing use
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
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This Item | H4281 | 14610C | 14621C |
---|---|---|---|
sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable |
form liquid | form liquid | form liquid | form liquid |
components glucose: 2.84 g/L | components HEPES: yes | components glucose: 3.45 g/L | components NaHCO3: 1.6 g/L |
storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Features and Benefits
Developed to meet the needs of biotechnology manufacturing, this medium supports rapid initial cell growth and high levels of antibody expression. It also supports high cell densities at high viability for extended periods without the use of animal-derived proteins.
Caution
Reconstitution
Legal Information
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.
Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.
The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.
Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.
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