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H4409

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EX-CELL® CD Hybridoma Medium, Chemically Defined

Animal-component free, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for hybridoma, suitable for cell culture

Synonym(s):

Hybridoma Medium

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1 EA
₹75,396.40

About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

₹75,396.40


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Quality Level

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
H428114610C14621C
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

components

HEPES: yes
NaHCO3: yes
L-glutamine: no
phenol red: no
glucose: 2.9 g/L

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

components

NaHCO3: 1.6 g/L
HEPES: 7.5 mM
L-glutamine: no
glucose: 3.45 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Features and Benefits

This medium offers the advantages of reduced raw material costs and uniform lot-to-lot product consistency. In addition, it reduces the risk of contamination by adventitious agents associated with animal-derived materials, making it of particular interest for biopharmaceutical applications. Cells can be successfully transferred from serum-supplemented cultures to these medium with little or no adaptation.
Developed to meet the needs of biotechnology manufacturing, this medium supports rapid initial cell growth and high levels of antibody expression. It also supports high cell densities at high viability for extended periods without the use of animal-derived proteins.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.

Reconstitution

Prior to use medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    Articles

    Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

    Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

    The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

    Protocols

    Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

    Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

    Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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