H8889

Histopaque^®-1077 Hybri-Max^™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

• Synonym(s): Histopaque 1077
• UNSPSC Code: 12352201
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• technique(s): cell culture | hybridoma: suitable; single cell analysis: suitable
• impurities: endotoxin, tested
• density: 1.077 at 25 °C (relative density)(lit.)
• storage temp.: 2-8°C

Description

General description

Histopaque^®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm³. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)² typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque^®-1077 Hybri-Max^™ has been used to isolate:

• peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
• immune cells (lymphocytes) from peripheral blood
• PBMCs from blood

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves