I5131

IgG from sheep serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

• Synonym(s): Sheep IgG
• MDL number: MFCD00165834
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: sheep
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• grade: reagent grade
• Assay: ≥95% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• isotype: IgG
• solubility: saline: 1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.)
• storage temp.: 2-8°C

Description

General description

IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds.

Application

Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

IgG mediates transplacental transport and participates in the secondary immune response. IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy. It has the longest serum half life.

Analysis Note

Protein concentration should be determined after reconstitution by absorbance at 280 nm (E_1%/280 = 14).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves