I5273

Interleukin-5 human

recombinant, expressed in baculovirus infected Sf21 cells, ≥97% (SDS-PAGE), lyophilized powder, suitable for cell culture

• Synonym(s): IL-5
• MDL number: MFCD00163946
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.1-0.5 ng/mL EC₅₀
• mol wt: 32-34 kDa
• packaging: pkg of 5 μg
• concentration: 0.5-10 ng/mL
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P05113
• storage temp.: −20°C
• Gene Information: human ... IL5(3567)

Description

Biochem/physiol Actions

IL-5 (interleukin-5) is a maturation and differentiation factor for eosinophils. Thus, it is associated with allergy and related disorders. IL-5 association with its receptor causes activation of MEK/ERK (mitogen activated protein kinase kinase/extracellular-regulated kinase), p38 MAPK (mitogen activated protein kinase) and JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathways.

Interleukin-5 is a product of activated T lymphocytes and exhibits activity on eosinophils, B cells and thymocytes. Synonyms or biological activities attributed to IL-5 include: EDF (eosinophil differentiating factor), BCGF-II (B cell growth factor-II), TRF (T cell replacing factor), IgA-EF (IgA enhancing factor) and EO-CSF (eosinophil colony stimulating factor). Natural IL-5 is a 45-50 kDa dimeric glycoprotein, although glycosylation is not required for activity. Human and mouse IL-5 exhibit homology at the nucleotide and amino acid levels and show species cross-reactivity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg bovine serum albumin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves