Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

KSPQ12012

KiCqStart® SYBR® Green Primers

Predesigned primers for gene expression analysis

Synonym(s):

Pre-designed RT-qPCR Primers, SYBR Green RT-qPCR Primers

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200

storage temp.

−20°C

General description

Overview
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.

1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.

To learn more about KiCqStart Primers visit our Frequently Asked Questions section.

Specifications
Design characteristics
  • GC Content: 20 - 80%2
  • Melting Temperature: 55.0 ± 5 ºC
  • Secondary Structure: None
  • Excessive 3′ Clamping: Minimal
  • Homology: None

Oligo characteristics
  • Molecule: DNA (all sequences verified by mass spec QC); no modifications
  • Contents: 1 forward and 1 reverse primer
  • Length: 18 - 24 bases2
  • Purification: RP cartridge
  • Format: 3 OD minimum yield, dry; 1 oligonucleotide per tube

Sequences are provided at the time of shipment.

295% of primer pairs fall within these specifications

Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
  • 1: The middle of one primer overlaps an exon splice site
  • 2: The 5′ or 3′ end of one primer overlaps an exon splice site
  • 3: The amplicon, but neither primer, overlaps an exon splice site
  • 4: Primer overlap is not possible for various reasons, e.g. the gene has only one exon (primer pairs with this rank may lead to co-amplification of genomic DNA).

Application

Product use: gene expression analysis

Features and Benefits

KiCqStart Primers are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective SYBR Green I RT-qPCR Assay.
Benefits include:
  • Target analysis is done
  • Design characteristics are set
  • Oligo characteristics are set
  • Easy to order

Physical properties

Properties include:
  • Available species: human, mouse, rat, zebrafish, cow, horse, Arabidopsis, chicken, dog, rabbit, pig, cat, rice, soybean, rhesus, Drosophila, C. elegans, corn, X. laevis, Guinea Pig, and Chinese Hamster.
  • Components: two primers, one oligo per tube
  • Shipping condition: dry, ambient temperature
  • Storage condition: dry or wet single-use aliquots

Other Notes

Frequently Asked Questions section is available for this product.

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
SYBR is a registered trademark of Life Technologies

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mara Ribeiro Almeida et al.
Molecular nutrition & food research, 60(7), 1615-1624 (2016-03-05)
Vitamin B6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at
Agata Nyga et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 107(5), 1352-1362 (2018-09-28)
We aimed to find a biomarker for patients with adverse reaction to metal debris (ARMD) due to a metal-on-metal (MoM) hip implant. First, we compared molecular markers of hypoxia-inducible factor (HIF) pathway activation (BNIP3, GLUT1, HO1, VEGF, and HIF1A) and
Helen Heath et al.
The EMBO journal, 27(21), 2839-2850 (2008-10-17)
The 11-zinc finger protein CCCTC-binding factor (CTCF) is a highly conserved protein, involved in imprinting, long-range chromatin interactions and transcription. To investigate its function in vivo, we generated mice with a conditional Ctcf knockout allele. Consistent with a previous report
G R Sharath Babu et al.
Frontiers in pharmacology, 8, 868-868 (2017-12-13)
Pelargonidin chloride (PC) is one of the major anthocyanin found in berries, radish and other natural foods. Many natural chemopreventive compounds have been shown to be potent inducers of phase II detoxification genes and its up-regulation is important for oxidative
Saravanan Ayyadurai et al.
Journal of leukocyte biology, 102(6), 1299-1312 (2017-07-08)
Life stress is a major risk factor in the onset and exacerbation of mast cell-associated diseases, including allergy/anaphylaxis, asthma, and irritable bowel syndrome. Although it is known that mast cells are highly activated upon stressful events, the mechanisms by which
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service