L2542

LB Broth (Miller)

Liquid microbial growth medium

• Synonym(s): Luria Broth Miller, Luria-Bertani broth, Miller
• UNSPSC Code: 41106200
• NACRES: NA.85

Properties

• grade: Molecular Biology
• Quality Level: 400
• sterility: sterile; 0.2 μm filtered
• form: liquid
• technique(s): microbiological culture: suitable
• pH: 6.5-7.2
• application(s): microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

• non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
• selective cultivation when appropriate antibiotics are added.
• the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:

• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

Components

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves