L2879
Lysozyme chloride form from chicken egg white
Grade VI, ≥35,000 units/mg protein (E1%/282)
Synonym(s):
Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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About This Item
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biological source
chicken egg white
Quality Level
type
Grade VI
form
lyophilized powder
specific activity
≥35,000 units/mg protein (E1%/282)
mol wt
~14.3 kDa
composition
Protein, ≥80%
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
cell analysis
storage temp.
−20°C
Related Categories
General description
Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).
Application
Lysozyme chloride form from chicken egg white has been used:
- as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
- in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
- in viscometric assay for determining lysozyme activity
Biochem/physiol Actions
Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.
Unit Definition
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Physical form
Lyophilized powder containing sodium chloride and sodium acetate
Preparation Note
3× Crystallized
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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