L6632

Lipoxidase from Glycine max (soybean)

Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

• Synonym(s): Linoleate:oxygen oxidoreductase, Lipoxygenase
• CAS Number: 9029-60-1
• EC Number: 1.13.11.12 (BRENDA, IUBMB)
• EC Number: 232-853-1
• MDL number: MFCD00131527
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type V
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: 500,000-1,000,000 units/mg protein
• mol wt: 94 kDa
• storage temp.: 2-8°C

Description

Application

Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ solution, pH approx. 6.0

Preparation Note

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves