L7651
Lysozyme from chicken egg white
lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
Recommended Products
grade
Molecular Biology
form
lyophilized powder
specific activity
≥40,000 units/mg protein
mol wt
single-chain 14.3 kDa
composition
protein, ≥90%
UniProt accession no.
storage temp.
−20°C
Gene Information
chicken ... LYZ(396218)
Looking for similar products? Visit Product Comparison Guide
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Lysozyme hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine, a polysaccharide backbone of peptidoglycans in the cell wall structure of many microorganisms. This is particularly useful for lysing Gram-positive and Gram-negative bacteria for subsequent nucleic acid extraction.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Features and Benefits
- Highly purified by repeated crystallization and dialysis
- Each lot is use-tested for isolation of plasmid DNA from E. coli
Unit Definition
One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Physical form
Lyophilized powder, essentially salt-free
Preparation Note
3× crystallized
replaced by
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service