L8777

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

• Synonym(s): PWM, Pokeweed mitogen
• MDL number: MFCD00161973
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• sterility: aseptically filled
• Quality Level: 200
• product line: BioReagent
• form: lyophilized powder
• potency: ≤0.3 μg/mL mitogenic activity
• technique(s): cell culture | mammalian: suitable
• solubility: PBS: 1 mg/mL
• storage temp.: 2-8°C

Description

General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

Biochem/physiol Actions

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.

PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Quality

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P280 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves