M6159

α₂-Macroglobulin from human plasma

BioUltra, ≥98% (SDS-PAGE)

• Synonym(s): α₂-M
• CAS Number: 1631137-21-7
• MDL number: MFCD00130472
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: human plasma
• Quality Level: 200
• product line: BioUltra
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• mol wt: ~720 kDa (four glycoprotein subunits)
• composition: Protein, 15-30% biuret
• technique(s): inhibition assay: suitable
• solubility: water: soluble 10 mg protein/mL, clear, colorless
• UniProt accession no.: P01023
• storage temp.: −20°C
• Gene Information: human ... A2M(2)

Description

Application

α₂-Macroglobulin was used to study impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-β which is an early biomarker for mild cognitive impairment preceding Alzheimer′s disease. It was also used in the control of the classical and the MBL pathway of complement activation.

Inhibits all classes of endoproteases by forming a complex with the protease. When the protease cleaves the macroglobulin "bait" sequence, the macroglobulin rearranges and traps the protease.

Biochem/physiol Actions

α₂-Macroglobulin (α₂M) is a multifunctional protein that is a broad spectrum protease inhibitor. α₂M is a large homotetrameric glycoprotein (720 kDa) that is connected by disulfide-linked dimers which non-covalently interact to give the quaternary structure. α₂M is found in normal plasma at a concentration of 220-230 mg/dl accounting for 3-5% of the total plasma protein.

α₂-Macroglobulin is found abundantly in plasma and interstitial fluids. The protease-α₂-M balance plays an important role in mediating inflammatory tissue destruction. Serum levels of α₂-M and protease-α₂-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis, and other inflammatory diseases, and oxidant inactivation of α₂-M may contribute to tissue destruction during inflammation.

Serum levels of α₂-Macroglobulin (α₂-M) and protease-α₂-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis and other inflammatory diseases. It is hypothesized that the oxidant inactivation of α₂-M contributes to tissue destruction in inflammation.

Packaging

Package size based on protein content

Physical form

Lyophilized from 0.02 M Tris, 0.13 M glycine, pH 8.0, and 0.08 M trehalose

Analysis Note

Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable