M9695

Matrix Metalloproteinase-12, Catalytic Domain human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MMP-12, Macrophage Elastase
• MDL number: MFCD02688053
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: calculated mol wt 20.3 kDa
• UniProt accession no.: P39900
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MMP12(4321)

Description

General description

Matrix metalloproteinase-12 (MMP-12) is also called as macrophage metalloelastase and is secreted by inflammatory macrophages. It is synthesized as a zymogen and then activated by cleaving the propeptide domain. The MMP-12 gene is localized on chromosome 11.

Biochem/physiol Actions

MMP-12 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

Matrix metalloproteinase-12 (MMP-12) breaks down proteoglycans, collagen type IV and laminin. Its main substrate is elastin.

Unit Definition

One unit equals 100 pmol/min at 37 °C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt as substrate.

Physical form

Solution in 50 mM Tris, 5 mM calcium chloride, 500 mM sodium chloride, 20 μM zinc chloride, 0.5% Brij^® L23, and 30% glycerol, pH 9.5.

Legal Information

Brij is a registered trademark of Croda International PLC

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves