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Key Documents

MBD0002

Sigma-Aldrich

Microbial DNA standard from Proteus mirabilis

Suitable for PCR, sequencing and NGS

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24

Quality Level

form

liquid

concentration

10 ng/μL

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

ambient

storage temp.

−20°C

Related Categories

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Proteus mirabilis genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Proteus mirabilis is a gram negative facultative anaerobic rod-shaped bacterium. Bacteria of the genus Proteus of the family Enterobacteriaceae are opportunistic human pathogens responsible for wound and burn infections as well as skin, eye, ear, nose, throat, urinary tract, and gastrointestinal infections and bacteremias.2 The most common infection involving P. mirabilis occurs when the bacteria, which is a member of the natural intestinal flora, moves to the urethra and urinary bladder causing urinary tract infection. The outer-membrane lipopolysaccharide (LPS) is considered an important virulence factor of Proteus.3 Arabski et al. suggest that the immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.4 A possible correlation between the abundance of P. mirabilis in the intestine and obesity was suggested recently.5

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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