N2415

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

• Synonym(s): PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase
• CAS Number: 9030-21-1
• EC Number: 2.4.2.1 (BRENDA, IUBMB)
• EC Number: 232-857-3
• MDL number: MFCD00131739
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥80% protein basis (biuret)
• form: lyophilized powder
• specific activity: ≥10 units/mg protein
• sequence note: MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
• UniProt accession no.: P0ABP8
• storage temp.: −20°C
• SMILES string: [n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
• InChI: 1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
• InChI key: MRWXACSTFXYYMV-FDDDBJFASA-N
• Gene Information: Escherichia coli ... deoD(945654)

Description

Application

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.

Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Biochem/physiol Actions

Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.

Unit Definition

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO₄, pH 7.4 solution.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable