N3755

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

• Synonym(s): Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease
• CAS Number: 9013-53-0
• EC Number: 3.1.31.1 (BRENDA, IUBMB)
• EC Number: 232-748-0
• MDL number: MFCD00131731
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Staphylococcus aureus
• Quality Level: 300
• form: lyophilized powder
• specific activity: 100-300 units/mg protein
• composition: Protein, ≥40% E1%/280 (, Balance primarily sodium citrate)
• technique(s): DNA purification: suitable
• suitability: suitable for molecular biology
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Description

Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.

Hydrolyzes 5′-phosphodiester bonds of DNA.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Other Notes

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves