O3008

Oleic Acid-Albumin from bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): Oleic acid-BSA complex
• MDL number: MFCD00284022
• UNSPSC Code: 12352211
• NACRES: NA.75

Properties

• biological source: bovine
• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• composition: Oleic acid, 2 mol/mol albumin
• concentration: 100 mg/mL BSA in DPBS; 90-120 mg/mL protein (biuret)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C

Description

Application

Oleic Acid-Albumin from bovine serum has been used: as a component of the Dulbecco′s modified Eagle′s medium (DMEM) to study adipogenic differentiation in chicken embryonic fibroblast cell lines, to study its effects on benign prostate epithelial cells as a component of the cell culture medium to quantify triglycerides in human epithelial colorectal adenocarcinoma cells

Biochem/physiol Actions

Fatty Acid-Albumin - complexes are used as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Serum proteins may help in the release of beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) shows a positive effect on the growth of various cells.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves