P3243

Phosphodiesterase I from Crotalus adamanteus venom

vial of ≥100 units, Purified

• Synonym(s): 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
• CAS Number: 9025-82-5
• EC Number: 3.1.4.1 (BRENDA, IUBMB)
• EC Number: 232-806-5
• MDL number: MFCD00131883
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Crotalus adamanteus venom
• Quality Level: 200
• form: solid
• quality: Purified
• specific activity: ≥20.0 unit/mg solid
• packaging: vial of ≥100 units
• storage temp.: −20°C

Description

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Unit Definition

One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9

Preparation Note

Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

Reconstitution

For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable