P4005

Protamine

solid

• Synonym(s): Protamine
• CAS Number: 9012-00-4
• EC Number: 232-726-0
• MDL number: MFCD00132089
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Protamine from salmon, Grade IV, Histone, free(Millon test)
• biological source: fish (salmon)
• Quality Level: 200
• type: Grade IV
• form: solid
• impurities: Histone, free (Millon test)
• solubility: H₂O: soluble, clear to slightly hazy, colorless to faintly yellow
• storage temp.: −20°C

Description

General description

Protamines are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. Salmon fish contains around 15 genes encoding protamine. It is found to be localized to the sperm head.

Application

Protamine from salmon has been used to avoid in vitro AT complex formation in samples from dogs pre-treated with heparin. It has also been used to study the influences of external potential on adsorption of various proteins to a metal surface.

Biochem/physiol Actions

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamines are found to displace histones in DNA during spermatogenesis in animals and plants. Protamine is useful as a heparin neutralizer during heart surgery, dialysis and in many other clinical procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves