P4300
Pectinase from Rhizopus sp.
suitable for plant cell culture, BioReagent, crude powder
Synonym(s):
Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
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About This Item
Recommended Products
product line
BioReagent
form
crude powder
specific activity
400-800 units/g solid
technique(s)
cell culture | plant: suitable
storage temp.
−20°C
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General description
Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Unit Definition
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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