P6534

Phospholipase A₂ from porcine pancreas

ammonium sulfate suspension, ≥600 units/mg protein

• Synonym(s): Lecithinase A, PLA₂, Phosphatidylcholine 2-acylhydrolase
• CAS Number: 9001-84-7
• EC Number: 3.1.1.4 (BRENDA, IUBMB)
• EC Number: 232-637-7
• MDL number: MFCD00131890
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: ammonium sulfate suspension
• Quality Level: 200
• specific activity: ≥600 units/mg protein
• UniProt accession no.: P00592
• storage temp.: 2-8°C
• Gene Information: pig ... PLA2G1B(445525)

Description

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 5.5

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable