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Sigma-Aldrich

GlycoProfile™ I, In-Gel Deglycosylation Kit

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20 μG
MYR 741.00
50 μG
MYR 1,323.00

MYR 741.00

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20 μG
MYR 741.00
50 μG
MYR 1,323.00

About This Item

EC Number:
232-615-7
UNSPSC Code:
12352200
NACRES:
NA.32

MYR 741.00

Check Cart for Availability
Request a Bulk Order
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shelf life

≥1 yr at 2‑8 °C

Quality Level

200

storage temp.

2-8°C

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This Item
SAB1410438SAB1402675SAB1300589
Monoclonal Anti-NUP133, (C-terminal) antibody produced in mouse clone 4F6, purified immunoglobulin, buffered aqueous solution

SAB1405159

Monoclonal Anti-NUP133, (C-terminal) antibody produced in mouse

Anti-NUP62 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

SAB1410438

Anti-NUP62 antibody produced in rabbit

Monoclonal Anti-NUP43, (C-terminal) antibody produced in mouse clone 2G5, purified immunoglobulin, buffered aqueous solution

SAB1402675

Monoclonal Anti-NUP43, (C-terminal) antibody produced in mouse

Anti-NUP54 (center) antibody produced in rabbit saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution

SAB1300589

Anti-NUP54 (center) antibody produced in rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

saturated ammonium sulfate (SAS) precipitated

clone

4F6, monoclonal

clone

polyclonal

clone

2G5, monoclonal

clone

polyclonal

Gene Information

human ... NUP133(55746)

Gene Information

human ... NUP62(23636)

Gene Information

human ... NUP43(348995)

Gene Information

human ... NUP54(53371)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

Application

The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Principle

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantity

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 USDS
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
  • Trypsin Solubilization Reagent
  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiaeSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBV6157
    037K6810
    016K6837
    065K6251
    023K9176

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