R7876

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

• Synonym(s): Transfer RNA, tRNA
• CAS Number: 9014-25-9
• MDL number: MFCD00132200
• UNSPSC Code: 12352106
• NACRES: NA.55

Properties

• grade: Molecular Biology
• Quality Level: 300
• type: Type V
• form: lyophilized powder
• specific activity: 15-19 units/mg solid
• storage condition: desiccated
• solubility: H₂O: ~2 mg/mL, clear to hazy
• storage temp.: 2-8°C

Description

General description

Wheat germ tRNA contains many different specific tRNA molecules.

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.

Suitable for use as a carrier in nucleic acid purification and precipitation.

Unit Definition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Preparation Note

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves