R7884

Ribonuclease B from bovine pancreas

BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

• Synonym(s): RNase B
• CAS Number: 9001-99-4
• EC Number: 3.1.27.5 (BRENDA, IUBMB)
• EC Number: 232-646-6
• MDL number: MFCD00132184
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: bovine pancreas
• Quality Level: 200
• product line: BioReagent
• Assay: ≥80% (SDS-PAGE)
• form: powder
• specific activity: ≥50 Kunitz units/mg protein
• concentration: ≥60%
• technique(s): cell based assay: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• foreign activity: protease ≤0.001 units/mg solid
• storage temp.: −20°C
• SMILES string: [nH]1cncc1CC(NC(=O)CCN)C(=O)O
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• InChI key: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

Application

Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.

Biochem/physiol Actions

Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.

Packaging

Package size based on protein content

Preparation Note

Purified by affinity chromatography

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves