RAB0817

Mouse Ins1 / Insulin-1 ELISA Kit

for serum, plasma and cell culture supernatants

• Synonym(s): Insulin
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 5 μIU/mL; standard curve range: 6.25-400 μIU/ml
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Ins1(16333)

Description

General description

The antibody pair provided in this kit recognizes Mouse Insulin 1.

Application

Mouse Ins1 / Insulin-1 ELISA Kit has been used to measure changes in serum insulin in male Sprague-Dawley rats with chemically induced diabetes.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Knockout of Ins-1 and Ins-2 leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABELADC ELISA 1X Assay/Sample Diluent Buffer C (Item L)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable