S1629

Sulfatase from Aerobacter aerogenes

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

• Synonym(s): Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
• CAS Number: 9016-17-5
• EC Number: 3.1.6.1 (BRENDA, IUBMB)
• MDL number: MFCD00132399
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type VI
• Quality Level: 200
• form: buffered aqueous glycerol solution
• specific activity: 2-5 units/mg protein (biuret)
• mol wt: ~41 kDa
• concentration: 10-20 units/mL
• foreign activity: β-Glucuronidase ≤10 U/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Application

Sulfatase from Aerobacter aerogenes has been used:

• as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
• in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
• to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Biochem/physiol Actions

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable