S9076

Superoxide Dismutase I human

recombinant, expressed in E. coli

• EC Number: 1.15.1.1 (BRENDA, IUBMB)
• EC Number: 232-943-0
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: 31 kDa
• technique(s): cell culture | mammalian: suitable
• color: white
• application(s): detection
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SOD1(6647)

Description

General description

Research Area: Cell Signaling
Cu/Zn superoxide dismutase (SOD1) is an intracellular antioxidant enzyme. A mature, functional human SOD1 is a relatively small (32 kDa) homodimeric metalloprotein.

Application

Superoxide Dismutase I human has been used to treat THP-1 (human leukemia monocytic cell line) or human primary macrophage cells to confirm signal specificity for superoxide.

Biochem/physiol Actions

Cu/Zn superoxide dismutase (SOD1) regulates basal levels of oxidative stress arising from the production of mitochondrial and cytosolic superoxide (O2 .−). Its high cytosolic abundance makes it unique from the other two human superoxide dismutases. SOD1 was believed to be a copper (Cu) storage protein, however, the crucial role of SOD1 is to act as an intracellular antioxidant. SOD1 also initiates gene transcription following exposure to neurotoxic stimuli and modulates signal transduction pathways involving reactive oxygen species (ROS). However, it is also implicated in multiple molecular mechanisms of cytotoxicity, contributing to pathology in diseases such as heart failure, cancer, diabetes, Down′s syndrome, amyotrophic lateral sclerosis (ALS), and Parkinson′s disease.

Physical properties

Recombinant Superoxide Dismutase I is fully biologically active when compared to standard.

Reconstitution

Reconstitute in H₂O to a concentration of ≥100 μg/ml. The solution can then be diluted into other aqueous buffers.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable