SAB4200119

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

2-4 mg/mL, clone 6F7, purified immunoglobulin

• Synonym(s): Anti-ddddk, Anti-dykddddk
• UNSPSC Code: 12352203
• NACRES: NA.32

Properties

• biological source: rat
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6F7, monoclonal
• form: buffered aqueous solution
• species reactivity: all
• concentration: 2-4 mg/mL
• technique(s): western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.

The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.

Immunogen

purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).

Application

Learn more product details in our FLAG^® applications portal.

Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable