Skip to Content
Merck
All Photos(1)

Key Documents

SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

Synonym(s):

Sucrose Test Kit

Sign Into View Organizational & Contract Pricing


About This Item

EC Number:
UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (sucrose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Kit Components Only

Product No.
Description

  • glucose assay reagent 50 mL

  • sucrose assay reagent 1 mL/vial

  • sucrose standard 500 mg

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service