Skip to Content
Merck

Skip To

SRP3119

Sigma-Aldrich

NANOG human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sign Into View Organizational & Contract Pricing

Select a Size

100 MG
₹8,987.00
500 MG
₹35,530.00

₹8,987.00


Please contact Customer Service for Availability

Select a Size

Change View
100 MG
₹8,987.00
500 MG
₹35,530.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

₹8,987.00


Please contact Customer Service for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

34.5 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

General description

NANOG gene is localized to human chromosome 12p13.31 and has multiple pseudogenes. This gene is composed of four exons, three introns, and a 915bp open reading frame (ORF). The encoded protein was first discovered in ESCs (embryonic stem cells) and is a divergent homeobox domain protein.[1][2] NANOG protein is composed of 305 amino acids and a central homeodomain (HD). This HD is composed of an unstructured N-terminal arm, a bundle of three α-helices (H1–H3) interlinked by loops.[3]
Recombinant human Nanog is a 34.7kDa protein, which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion.

Biochem/physiol Actions

NANOG is one of the central genes regulating self-renewal and pluripotency capacities of embryonic stem cells (ESC). Constitutive expression of this gene prevents the differentiation of ESCs, and along with SOX2 (SRY-box 4) and OCT-4 (octamer-binding transcription factor 4), it regulates pluripotency-related gene expression and maintains the pluripotency of ESCs. During ESC differentiation both these genes are down-regulated, and gene profiling shows that the expression of NANOG is uniformly high in ESC lines. There is significant similarity between ESC and carcinoma in situ testis (CIS), including the high expression of NANOG. It is novel marker for testicular CIS and germ cell tumors. NANOG shows abnormal expression in a variety of human cancers, such as carcinomas of the brain, oral cavity, head and neck, breast, lung, liver, pancreas, kidney, gastric, cervix, ovary, prostate, and colon. This expression is also related to treatment resistance and poor survival of cancer patients.[1][2]

Sequence

SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV

Physical form

Lyophilized with 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    María del Carmen Córdoba-Pedregosa et al.
    Journal of experimental botany, 56(412), 685-694 (2004-12-08)
    Onions (Allium cepa L.) treated with external ascorbic acid or with the immediate precursor of its synthesis L-galactono-gamma-lactone show a stimulated elongation rate of the roots and an increase in the number of new radicles appearing at the bulb base.
    Yongshun Gao et al.
    Journal of experimental botany, 62(10), 3647-3657 (2011-03-23)
    Ascorbate (AsA) is a redox buffer and enzyme cofactor with various proposed functions in stress responses and growth. The aim was to identify genes whose transcript levels respond to changes in leaf AsA. The AsA-deficient Arabidopsis mutant vtc2-1 was incubated
    J Leipner et al.
    Planta, 210(6), 964-969 (2000-06-29)
    Infiltrating detached maize (Zea mays L.) leaves with L-galactono-1,4-lactone (L-GAL) resulted in a 4-fold increase in the content of leaf ascorbate. Upon exposure to high irradiance (1000 mumol photons m-2 s-1) at 5 degrees C, L-GAL leaves de-epoxidized the xanthophyll-cycle
    C M Spickett et al.
    Free radical biology & medicine, 28(2), 183-192 (2001-04-03)
    This study investigated the ability of the yeast Saccharomyces cerevisiae to synthesize ascorbate and its 5-carbon analogue erythroascorbate from a variety of precursors, and their importance as antioxidants in this organism. Studies of ascorbate and analogues in micro-organisms have been
    Vincent R Franceschi et al.
    Plant physiology, 130(2), 649-656 (2002-10-12)
    L-Ascorbic acid (AsA) was found to be loaded into phloem of source leaves and transported to sink tissues. When L-[(14)C]AsA was applied to leaves of intact plants of three different species, autoradiographs and HPLC analysis demonstrated that AsA was accumulated

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service