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SRP3297

Sigma-Aldrich

ADIPONECTIN mouse

recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

Acrp-30, Apm-1, GBP-28

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
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biological source

mouse

recombinant

expressed in Hi-5 Insect cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

4.0-6.0 μg/mL ED50

mol wt

35.0 kDa

packaging

pkg of 25 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

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S5192SML1555SML2534
JSH-23 &#8805;98% (HPLC), solid

J4455

JSH-23

SB 222200 &#8805;98% (HPLC), solid

S5192

SB 222200

DJ-V-159 &#8805;98% (HPLC)

SML2534

DJ-V-159

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

form

solid

form

solid

form

powder

form

powder

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage condition

protect from light

storage condition

-

storage condition

desiccated

storage condition

-

solubility

DMSO: >10 mg/mL

solubility

DMSO: soluble ~28 mg/mL, H2O: insoluble

solubility

H2O: 20 mg/mL, clear

solubility

DMSO: 2 mg/mL, clear

Biochem/physiol Actions

Adiponectin is an adipose-derived secreted protein containing 236 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-a and class A scavenger receptor in macrophages). Recombinant adiponectin is a multimeric glycoprotein containing amino acids Val-21 to Asn-247 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions.

Physical form

Lyophilized from 20 mM Tris, pH 8.5 + 75 mM L-Arginine.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/mL. Note: Allow the reconsituted vial to sit at room temperature for 30 minutes before use.Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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