T1408

holo-Transferrin bovine

• Synonym(s): Siderophilin, Siderophilin, iron-saturated
• CAS Number: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00131337
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine
• Quality Level: 200
• Assay: 97-100% (agarose gel electrophoresis)
• form: powder
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: Q29443
• storage temp.: 2-8°C
• Gene Information: bovine ... TF(280705)

Description

General description

Holo-Transferrin is an iron transporting glycoprotein and belongs to metalloprotein class. The gene encoding transferrin is mapped to bovine chromosome 1.

Application

Holo-Transferrin bovine has been used:

• as a RPMI-1640 medium supplement for culturing cancer cell lines
• as a component of visceral fat differentiation medium for the pre-adipocytes primary cell culture
• to test the influence of iron-saturation complex on the growth of Malassezia pachydermatis
• as a standard for strong anion exchange (SAX) reactor measurements

Biochem/physiol Actions

Holo-Transferrin (holo-Tf) or the iron bound transferrin functions as an iron sequester and is crucial for defense against microbial infections. Transferrin is used as a model system for understanding the kinetics and dynamics of protein structure based interactions. It is a potential candidate for delivery studies for its ability to cross the blood-brain barrier. The interaction of holo-Tf with thrombin is implicated in the pathogenesis of intracerebral hemorrhage. Polymorphisms in the bovine transferrin gene has led to the generation of 10 variant transferrin protein.

Studies show that treatment of breast cancer cells with holo-transferrin in combination with dihydroartemisinin results in increased tumor cell death due to elevated levels of intracellular ferrous iron.

Analysis Note

Purity by agarose gel electrophoresis.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves