T3038
Trizma® hydrochloride solution
1 M, BioReagent
Synonym(s):
Tris hydrochloride solution
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About This Item
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grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
product line
BioReagent
form
solution
concentration
1 M
impurities
DNase, RNase, NICKase and protease, none detected
pH
8.0
application(s)
agriculture
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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General description
Trizma® hydrochloride solution is a useful biological buffer.
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride solution may be used in the following studies:
- As buffer for the 2-D electrophoresis of rat fibroblast cell.
- As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
- Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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