T8665

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

• Synonym(s): TMB
• UNSPSC Code: 12352204
• NACRES: NA.83

Properties

• Product Name: 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate
• Quality Level: 300
• form: liquid
• storage condition: protect from light
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H₂SO₄, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Caution

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Physical form

Ready-to-use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H226,H301 + H311 + H331,H319,H370
• Precautionary Statements: P210 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1
• Target Organs: Eyes,Central nervous system
• WGK: WGK 2