W0138

Western Blocker^™ Solution

for HRP detection systems

• Synonym(s): Western blot blocking solution, blocking buffer
• UNSPSC Code: 41105329
• NACRES: NA.25

Properties

• Quality Level: 200
• shelf life: ≥2 yr
• storage temp.: 2-8°C

Description

General description

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Application

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.

Features and Benefits

• Does not inhibit weak HRP signals, as many milk-based blockers do.
• Can be used both as a blocking component and as a probing component.
• Supplied ready-to-use; no preparative step is required.

Legal Information

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable