ZRB3163

Anti-TLR9/CD289 Antibody, clone 2L12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Toll-like receptor 9
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2L12, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 115.86 kDa; observed mol wt ~116 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey, primate
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal extracellular domain
• Protein ID accession no.: NP_059138
• UniProt accession no.: Q9NR96
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TLR9(54106)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2L12 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects TLR9/CD289. It targets an epitope within 16 amino acids from the N-terminal extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal extracellular domain of human TLR9/CD289.

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TLR9/CD289 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TLR9/CD289 in lysate from EL4 cells.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected TLR9/CD289 in Daudi cells.

Affinity Binding Assay: A representative lot of this antibody bound TLR9/CD289 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TLR9/CD289 in human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Toll-like receptor 9 (UniProt: Q9NR96; also known as CD289) is encoded by the TLR9 gene (Gene ID: TLR9) in human. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines. TLR9 is a single-pass type I membrane protein that serves as an innate immune sensor that recognizes microbial DNA. It exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand. TLR9 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 26-818), a short transmembrane domain (aa 819-839), and the cytoplasmic domain (aa 840-1032). The extracellular domain also contains 26 LRR repeat regions (aa 62-772). It is highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cell. It is also found in the basolateral region of gastric epithelial cells and high levels are detected in antrum and body mucosa. Five isoforms of TLR9 have been described that are produced by alternative splicing. Ligand recognition of TLR9 is shown to be strictly controlled to prevent unnecessary response to self-derived DNA such as mitochondrial DNA. Ligand-dependent TLR9 trafficking plays a critical role in type I interferon induction in macrophages and plasmacytoid dendritic cells. TLR9 is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Murakami, Y., et al. (2017). Sci. Rep. 7; 44042).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2