M9067

Anti-MRP1 antibody,Mouse monoclonal

clone QCRL-1, purified from hybridoma cell culture

• Synonym(s): Anti-Multidrug Resistance Associated Protein 1
• MDL number: MFCD01865488
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: QCRL-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~190 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: 10-20 μg/mL using cultured HeLa cells or HepG2 human hepatocellular carcinoma cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P33527
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ABCC1(4363)

Description

General description

Monoclonal Anti-MRP1 (mouse IgG1 isotype) is derived from the QCRL-1 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from mice immunized with non-denatured membrane preparations of human H69AR small cell lung cancer cell line, which highly expresses MRP1.

Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.

Specificity

Does not cross-react with the human MDR1 and MDR3, mouse MRP1, and human MRP2, MRP3, MRP4, MRP5, and MRP6 gene products. The antibody may cross-react with canine MRP1.

Immunogen

non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within amino acids 918-924 of human MRP1.

Application

Monoclonal Anti-MRP1 antibody produced in mouse has been used in:

• western blotting
• immunohistochemistry
• flow cytometry
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance to chemotherapy to cells in solid tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable