A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

• Sinonimo/i: AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase
• CAS Number: 37288-67-8
• Numero CE: 3.4.11.10 (BRENDA, IUBMB)
• Numero CE: 232-874-6
• Numero MDL: MFCD00166325
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Grado: Proteomics Grade
• Livello qualitativo: 300
• Stato: lyophilized powder
• Attività specifica: 50-150 units/mg protein
• PM: 29.5 kDa
• Composizione: Protein, ~40% biuret
• Solubilità: H₂O: soluble 0.9-1.1 mg/mL, clear, colorless
• Attività estranea: endopeptidase, essentially free
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

A zinc-containing enzyme.

Specificità

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Applicazioni

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Azioni biochim/fisiol

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Definizione di unità

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

Stato fisico

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Nota sulla preparazione

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H334,H335
• Consigli di prudenza: P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)