C4129

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

• Sinonimo/i: α-chymotrypsin A and B, alpha-chymotrypsin
• CAS Number: 9004-07-3
• Numero CE: 3.4.21.1 (BRENDA, IUBMB)
• Numero CE: 232-671-2
• Numero MDL: MFCD00130481
• Codice UNSPSC: 12352204
• eCl@ss: 42010112
• NACRES: NA.54

Proprietà

• Tipo: Type II
• Livello qualitativo: 300
• Stato: lyophilized powder
• Attività specifica: ≥40 units/mg protein
• PM: 25 kDa
• Composizione: protein, ≥85%
• Colore: white to off-white
• N° accesso UniProt: P00767
• applicazioni: diagnostic assay manufacturing
• Temperatura di conservazione: −20°C
• Informazioni sul gene: cow ... CTRB1(618826)

Descrizione

Applicazioni

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Azioni biochim/fisiol

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definizione di unità

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Nota sulla preparazione

Produced from 3× crystallized chymotrypsinogen

Risultati analitici

Protein determined by E1%/280

Altre note

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H315,H319,H334,H335,H400
• Consigli di prudenza: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Faceshields, Gloves