P7367

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE)

• Sinonimo/i: PNGase F
• CAS Number: 83534-39-8
• Numero CE: 3.5.1.52 (BRENDA, IUBMB)
• Numero MDL: MFCD18839214
• Codice UNSPSC: 12352204
• NACRES: NA.32

Proprietà

• Origine biologica: bacterial (Elizabethkingia meningoseptica)
• Livello qualitativo: 200
• Coniugato: (N-linked)
• Nome Commerciale: BioReagent
• Saggio: ≥95% (SDS-PAGE)
• Stato: powder
• Durata: ≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml); ≥1 yr at 2‑8 °C; Solution is stable for at least 3 freeze-thaw cycles
• PM: ~36 kDa
• Concentrazione: ≥300 units/mL; ≥50 units/mL
• pH ottimale: ~8.6
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Applicazioni

PNGase F from Elizabethkingia meningoseptica has been used:

• for de-N-glycosylation of Zika20virus E protein
• to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
• to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis

Used to deglycosylate protein.

Azioni biochim/fisiol

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Definizione di unità

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)