22178

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

• Synonym(s): 1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase
• CAS Number: 9012-54-8
• EC Number: 3.2.1.4 (BRENDA, IUBMB)
• EC Number: 232-734-4
• MDL number: MFCD00081510
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Aspergillus niger
• Quality Level: 100
• form: powder
• specific activity: ~0.8 U/mg
• color: off-white
• storage temp.: 2-8°C

Description

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Application

Cellulase from Aspergillus niger has been used:

• in the preparation of the standardized solution to determine absolute cellulase levels
• in phosphate-citric acid buffer for activity assay
• in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves