A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

• Synonym(s): AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase
• CAS Number: 37288-67-8
• EC Number: 3.4.11.10 (BRENDA, IUBMB)
• EC Number: 232-874-6
• MDL number: MFCD00166325
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• grade: Proteomics Grade
• Quality Level: 300
• form: lyophilized powder
• specific activity: 50-150 units/mg protein
• mol wt: 29.5 kDa
• composition: Protein, ~40% biuret
• solubility: H₂O: soluble 0.9-1.1 mg/mL, clear, colorless
• foreign activity: endopeptidase, essentially free
• storage temp.: −20°C

Description

General description

A zinc-containing enzyme.

Specificity

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves