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LPCG-501

Sigma-Aldrich

Lyophilized PEGylated Neutral Liposomes

HSPC:Chol:PEG2000-DSPE (65:30:5 molar ratio)

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.23
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form

lyophilized powder

composition

HSPC:Chol:PEG2000-DSPE (65:30:5 molar ratio)

color

white to off-white

mean particle size

100 nm

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1 of 4

This Item
67017-U67018-U67084-U
separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

L × I.D.

0.5 cm × 2.1 mm

L × I.D.

0.5 cm × 3 mm

L × I.D.

0.5 cm × 4.6 mm

L × I.D.

0.5 cm × 4.6 mm

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

cyano phase

particle size

5.0 μm

particle size

5.0 μm

particle size

5.0 μm

particle size

5.0 μm

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L10

product line

BIOshell

product line

BIOshell

product line

BIOshell

product line

BIOshell

General description

Lyophilized liposomes product series are freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. These products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays.
Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high-pressure extrusion through nano sized pore track etch membranes. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration.
To improve liposome stability and enhance their circulation times in the blood, a sterically stabilized, hydrophilic polymer, polyethylene glycol (PEG), has been shown to be the optimal choice for obtaining sterically stabilized liposomes. Using a Neutral Liposome with PEG will protect the liposomes from circulating proteins, improving their plasma clearance and enhancing their therapeutic effects.

Application

  • Liposomes are formed upon hydration of the lyophilized formulation. If the lyophilized liposomes are hydrated with solution containing a water-soluble drug, then a large percentage of the drug will stay outside of the liposomes and in non-encapsulated form. It is advised to use a micro dialysis cassette or a spin column using the right-side beads (depending on the size of your drug) and separate the drug encapsulated liposomes from free drug and perform the drug assay in order to calculate the encapsulation efficiency.
  • Lyophilized liposomes are mainly recommended to be used with drugs that have a short life in aqueous solution mainly due to hydrolysis. After adding the solution of the drug to lyophilized liposomes, the liposomes should be used immediately.
  • Lyophilized liposomes products should be used by scientists who understand liposome formulation and have the proper equipment in order to check the size, separate the non-encapsulated drug and do the proper assays.
  • Trehalose is used as a lyoprotectant in all freeze-dried liposome formulation. The size distribution after hydration of the freeze-dried formulation will be around 100 nm.
  • Freeze-dried liposomes should be kept at -20°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Kinetics of the reaction of ethanol and lauric acid catalyzed by deep eutectic solvent based on benzyltrimethylammonium chloride
Li Kun, et al.
The Canadian Journal of Chemical Engineering, 97(5), 1144-1151 (2019)
Selective oxidation of benzyl alcohol with hydrogen peroxide over reaction-controlled phase-transfer catalyst
Weng Z, et al.
Catalysis Communications, 8(10), 1493-1496 (2007)
Kathrin Müller et al.
Analytical and bioanalytical chemistry, 412(20), 4867-4879 (2020-03-05)
Matrix effects have been shown to be very pronounced and highly variable in the analysis of mobile chemicals, which may severely exacerbate accurate quantification. These matrix effects, however, are still scarcely studied in combination with hydrophilic interaction liquid chromatography (HILIC)
R Daniel Peluffo et al.
The Journal of general physiology, 123(3), 249-263 (2004-02-26)
The effects of organic quaternary amines, tetraethylammonium (TEA) chloride and benzyltriethylammonium (BTEA) chloride, on Na,K pump current were examined in rat cardiac myocytes superfused in extracellular Na(+)-free solutions and whole-cell voltage-clamped with patch electrodes containing a high Na(+)-salt solution. Extracellular
M De Amici et al.
Il Farmaco; edizione scientifica, 42(6), 409-424 (1987-06-01)
The results of a pharmacological investigation on a series of meta-substituted benzyltrimethylammonium salts possessing an antimuscarinic activity are reported. Correlative analysis shows that the pharmacodynamic activity is a function of the hydrophobic-lipophilic parameter associated with the substituent.

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