05-1135

Anti-PDGFR Antibody, clone CH-3

clone CH-3, from mouse

• Synonym(s): Platelet Derived Growth Factor Receptor Beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CH-3, monoclonal
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1

Description

General description

PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.

Immunogen

Recombinant Platelet-Derived Growth Factor Receptor

Application

ELISA: A previous lot of this antibody was used in ELISA.

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Growth Factors & Receptors

This Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR.

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates.

Target description

211 amino acids; 24043 Da

Linkage

Replaces: 04-397

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6

Storage and Stability

Maintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable