346774

Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides

Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate.

• Synonym(s): Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides
• CAS Number: 9001-40-5
• EC Number: 1.1.1.49 (BRENDA, IUBMB)
• MDL number: MFCD00081656
• UNSPSC Code: 12352202
• NACRES: NA.21

Properties

• Quality Level: 100
• form: lyophilized
• specific activity: ≥200 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• foreign activity: Creatine phosphokinase ≤0.002%; Phosphohexose isomerase ≤0.02%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate. The enzyme can use either NAD⁺ or NADP⁺ as an electron acceptor. Reaction velocity with NAD⁺ is ~1.8 times greater than that with NADP⁺. Note: 10,000 NADP units are equivalent to ~18,000 NAD units.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will reduce 1.0 µmol NADP per min at 30°C, pH 7.8.

Reconstitution

Reconstitute to 1 mg/ml in 5 mM glycine buffer, pH 8.0. Agitation of solution may cause precipitation.

Other Notes

Levy, H.R. 1989. Biochem. Soc. Trans. 17, 313.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable