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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
EDL H2AK15-4, monoclonal
species reactivity
Drosophila, human, mouse
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HIST1H2AG(8969)
General description
Histone H2A is one of four components of the core nucleosomal structure. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. The degree of ubiquitylation at DNA damage sites is regulated at multiple levels in order to generate proper DNA damage response. Uncontrolled ubiquitylation of histones can lead to unscheduled transcriptional silencing and generate defects in DNA damage checkpoint activation and cell cycle arrest. The RNF168 E3 ligase ubiquitylates H2A at K13, K15, which in turn triggers the recruitment of DNA damage response proteins, such as 53BP1 and BRCA1 to DNA break sites. USP51, a deubiquitinating enzyme, removes H2AK13, 15ub following DNA repair. Depletion of USP51 can result in elevated levels of H2AK13, 15ub on chromatin and cause delays in disassembly of proteins at DNA damage foci.
Specificity
Clone EDL H2AK15-4 specifically recognizes histone H2A monoubiquitylated at Lys15, but not unmodified H2A. Does not recognize di-ubiquitin, K63 and K27 linked di-ubiquitin species.
Immunogen
KLH-conjugated branched peptide corresponding to the ubiquitination site on human H2AK15.
Application
Anti-H2AK15ub, clone EDL H2AK15-4, Cat. No. MABE1119, is a highly specific mouse monoclonal antibody, that targets H2A ubiquitylated at Lys15 and has been tested in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected H2AK15ub in U2OS cells with or without RNF168 depletion (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).
Immunocytochemistry Analysis: A representative lot detected H2AK15ub in U2OS cells (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).
Immunocytochemistry Analysis: A representative lot detected H2AK15ub in U2OS cells (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).
Quality
Evaluated by Western Blotting in Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.
Western Blotting Analysis: 2 ug/mL of this antibody detected Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.
2 mg/mL
Western Blotting Analysis: 2 ug/mL of this antibody detected Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.
2 mg/mL
Target description
~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
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