B5050

Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse

clone 35928.11, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-BDNF
• MDL number: MFCD01633045
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 35928.11, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 8-25 μg/mL using human spinal cord; western blot: 1-2 μg/mL
• isotype: IgG1
• UniProt accession no.: P23560
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BDNF(627)

Description

General description

The antibody shows ~5% cross-reactivity with recombinant human β−NGF and recombinant rat β−NGF.

Immunogen

Recombinant human BDNF, expressed in Sf 21 cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) belongs to a family of secreted proteins called neurotrophins that includes, nerve growth factor (NGF), neurotrophin-3 (NT3) and NT4/5. BDNF is expressed in the developing and the mature brain. The downstream pathways promoted by BDNF include PI3K, MAPK and JAK/STAT. The most important role of BDNF is the regulation of synaptic transmission and plasticity, protein synthesis and phosphorylation of local proteins.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified from ascites fluid using protein A.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable