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G112080

Sigma-Aldrich

DMT-dG(tac) Phosphoramidite

configured for PerkinElmer, configured for Polygen

Synonym(s):

DMT-dG(tac) amidite

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1 G
₹23,155.00

About This Item

Empirical Formula (Hill Notation):
C52H62N7O9P
CAS Number:
Molecular Weight:
960.06
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.51

₹23,155.00


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type

for DNA synthesis

Quality Level

product line

Proligo Reagents

Assay

≥98% (31P-NMR)
≥98.0% (reversed phase HPLC)

form

powder

mol wt

960.06 g/mol

technique(s)

oligo synthesis: suitable

color

white to light yellow

solubility

acetonitrile: 0.2 M, clear

λ

conforms (UV/VIS Identity)

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SAB2101685HPA003360HPA003279
biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

General description

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Substitution of standard protecting groups with the labile TAC (tert.-butylphenoxyacetyl) protecting group results in ultra-fast and easydeprotection under mild conditions, suitable for oligonucleotides with baselabile monomers and reporters as well as in-situ synthesis schemes on glass surfaces.Key Features of TAC Chemistry
  • Deprotection of the TAC group is ultra-fast: complete deprotection inconcentrated ammonia occurs within 15 minutes at 55 °C or two hours atroom temperature
  • Compatible with the AMA deprotection reagent (a mixture of ≥25%ammonia in water with 40% aqueous methylamine I/I, v/v)
  • Highly soluble in acetonitrile. No need to add co-solvents such asdimethylformamide or methylene chloride
  • Suitable for the synthesis of oligomers with base-labile units e.g., dyes andmodifiers, because of less exposure to ammonia and the possibility ofroom temperature deprotection
  • No change is required in the reagents commonly used for DNA synthesis,except that Proligo′s Fast Deprotection Cap A solution is used instead ofCap A solution
  • The application of dA(tac) minimizes depurination and improves thequality of oligonucleotidesDMT-dG(tac) Phosphoramidite configured for Expedite and PolyGen® Synthesizers.

Legal Information

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
PolyGen is a registered trademark of PolyGen GmbH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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