L1044

Anti-Leukemia Inhibitory Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): CDF, D factor, DIA, Emfilermin, LIF, differentiation inhibitory activity
• MDL number: MFCD00283185
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): neutralization: suitable; western blot: 0.1-0.2 μg/mL
• UniProt accession no.: P09056

Description

General description

Leukemia inhibitory factor (LIF) is a pleotropic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes mouse leukemia inhibitory factor. It shows less than 5% cross-reactivity with recombinant human LIF.

Immunogen

purified, E. coli-derived recombinant mouse leukemia inhibitory factor.

Application

Anti-Leukemia Inhibitory Factor may be used in neutralization reactions at a working concentration of 0.5 ng/ml. For immunoblotting a concentration of 0.1-0.2 μg/ml is recommended.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves