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SAB4503763

Sigma-Aldrich

Anti-phospho-GSK3 α/β (pTyr279/216) antibody produced in rabbit

affinity isolated antibody

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50 μL
CA$147.00
250 μL
CA$297.00

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50 μL
CA$147.00
250 μL
CA$297.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

CA$147.00

Please contact Customer Service for Availability
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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

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Anti-Caspase 3, Active antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

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Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

biological source

rat

biological source

rabbit

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells

technique(s)

indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine, microarray: suitable, western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)

clone

polyclonal

clone

polyclonal

clone

14F7, monoclonal

clone

polyclonal

UniProt accession no.

Q6UXS9

UniProt accession no.

Q6UXS9

UniProt accession no.

Q920D5

UniProt accession no.

P42574

Immunogen

The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216.

Immunogen Range: 246-295

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    110012

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    Katherine E Deigan et al.
    Proceedings of the National Academy of Sciences of the United States of America, 106(1), 97-102 (2008-12-26)
    Almost all RNAs can fold to form extensive base-paired secondary structures. Many of these structures then modulate numerous fundamental elements of gene expression. Deducing these structure-function relationships requires that it be possible to predict RNA secondary structures accurately. However, RNA
    Scott P Hennelly et al.
    Nucleic acids research, 39(6), 2416-2431 (2010-11-26)
    Riboswitches are non-coding RNAs that control gene expression by sensing small molecules through changes in secondary structure. While secondary structure and ligand interactions are thought to control switching, the exact mechanism of control is unknown. Using a novel two-piece assay
    Matthew J Smola et al.
    Biochemistry, 54(46), 6867-6875 (2015-11-07)
    SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural
    Anthony M Mustoe et al.
    Cell, 173(1), 181-195 (2018-03-20)
    mRNAs can fold into complex structures that regulate gene expression. Resolving such structures de novo has remained challenging and has limited our understanding of the prevalence and functions of mRNA structure. We use SHAPE-MaP experiments in living E. coli cells to
    Jacob K Grohman et al.
    Journal of the American Chemical Society, 133(50), 20326-20334 (2011-12-01)
    Higher-order structure influences critical functions in nearly all noncoding and coding RNAs. Most single-nucleotide resolution RNA structure determination technologies cannot be used to analyze RNA from scarce biological samples, like viral genomes. To make quantitative RNA structure analysis applicable to
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