추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
culture supernatant
항체 생산 유형
primary antibodies
클론
3G4B2, monoclonal
종 반응성
human, canine, monkey
제조업체/상표
Upstate®
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NCL(4691)
일반 설명
Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
특이성
nucleolin
면역원
Nuclear matrix extract from MDCK cells
애플리케이션
Anti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.
품질
routinely evaluated by immunoblot on HeLa nuclear extract
표적 설명
100kDa
결합
Replaces: MABE466
물리적 형태
mouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%
분석 메모
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
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